Jacques P. Guyette, Ph.D.
Worcester Polytechnic Institute
Instructor in Surgery
During my graduate work, I studied several facets of cardiac regeneration from a biomedical engineering perspective, translating benchtop tissue engineering technologies to in vivo systems for therapeutic applications. For my dissertation work, I conditioned human marrow-derived mesenchymal stem cells with myocardial infarct cues in vitro to enhance the expression of cardiac genes and proteins, and then delivered these cells to rat infarcts in vivo to show improved cardiac function. In additional projects, I implanted biomaterials for surgical ventricular restoration of canine infarct models to evaluate myocardial regeneration, cardiac function, and material-host interactions. I also helped to develop new cell-seeded biomaterials for more efficient delivery for cell therapy, which can be used in a variety of applications. My background provides me with a working knowledge of stem cell science, cell & molecular characterization, biomaterial development, biomaterial-tissue interaction, surgical implantation models, and regenerative & functional outcomes.Bringing these skills to the Ott Lab, I work on the human heart regeneration project. Using the whole-organ decellularization technique, we are seeding new human iPS-derived cardiomyocytes onto decellularized cardiac matrix to regenerate functional human myocardium. In doing this, we study the biochemical and material properties of the decellularized myocardium, cell –matrix interactions of newly seeded human cardiomyocytes, biomimetic culturing conditions to mimic physiological parameters, and myocardial function.